Automated high-throughput mouse transsynaptic viral tracing using iDISCO+ tissue clearing, light-sheet microscopy, and BrainPipe

Transsynaptic viral tracing requires tissue sectioning, manual cell counting, and anatomical assignment, all of which are time intensive. We describe a protocol for BrainPipe, a scalable software for automated anatomical alignment and object counting in light-sheet microscopy volumes. BrainPipe can...

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Detalles Bibliográficos
Autores principales: Pisano, Thomas J., Hoag, Austin T., Dhanerawala, Zahra M., Guariglia, Sara R., Jung, Caroline, Boele, Henk-Jan, Seagraves, Kelly M., Verpeut, Jessica L., Wang, Samuel S.-H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038781/
https://www.ncbi.nlm.nih.gov/pubmed/35496792
http://dx.doi.org/10.1016/j.xpro.2022.101289

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038781/
https://www.ncbi.nlm.nih.gov/pubmed/35496792
http://dx.doi.org/10.1016/j.xpro.2022.101289

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