Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method

In chickens, primordial germ cells (PGCs) are effective targets for advanced genome editing, including gene knock-in. Although a long-term culture system has been established for chicken PGCs, it is necessary to select a gene-editing tool that is efficient and precise for editing the PGC genome whil...

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Detalles Bibliográficos
Autores principales: Ezaki, Ryo, Ichikawa, Kennosuke, Matsuzaki, Mei, Horiuchi, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Poultry Science Association 2022
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039151/
https://www.ncbi.nlm.nih.gov/pubmed/35528378
http://dx.doi.org/10.2141/jpsa.0210067

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039151/
https://www.ncbi.nlm.nih.gov/pubmed/35528378
http://dx.doi.org/10.2141/jpsa.0210067

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