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Optimization of stimulation and staining conditions for intracellular cytokine staining (ICS) for determination of cytokine-producing T cells and monocytes

Cell-mediated responses to immunological stimuli are often localised in inflammatory sites and involve a number of cell types. These responses can be functionally characterised at the single-cell level on the basis of the types of cytokines expressed either in whole blood or PBMCs. The ability to me...

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Detalles Bibliográficos
Autores principales: Mandala, Wilson, Harawa, Visopo, Munyenyembe, Alinane, Soko, Monica, Longwe, Herbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040130/
https://www.ncbi.nlm.nih.gov/pubmed/35492400
http://dx.doi.org/10.1016/j.crimmu.2021.10.002