Developing a reproducible protocol for culturing functional confluent monolayers of differentiated equine oviduct epithelial cells

We describe the development of two methods for obtaining confluent monolayers of polarized, differentiated equine oviduct epithelial cells (EOEC) in Transwell inserts and microfluidic chips. EOECs from the ampulla were isolated post-mortem and seeded either (1) directly onto a microporous membrane a...

Descripción completa

Detalles Bibliográficos
Autores principales: Leemans, Bart, Bromfield, Elizabeth G, Stout, Tom A E, Vos, Mabel, Van Der Ham, Hanna, Van Beek, Ramada, Van Soom, Ann, Gadella, Bart M, Henning, Heiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040661/
https://www.ncbi.nlm.nih.gov/pubmed/34962550
http://dx.doi.org/10.1093/biolre/ioab243

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040661/
https://www.ncbi.nlm.nih.gov/pubmed/34962550
http://dx.doi.org/10.1093/biolre/ioab243

Ejemplares similares