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Developing a reproducible protocol for culturing functional confluent monolayers of differentiated equine oviduct epithelial cells
We describe the development of two methods for obtaining confluent monolayers of polarized, differentiated equine oviduct epithelial cells (EOEC) in Transwell inserts and microfluidic chips. EOECs from the ampulla were isolated post-mortem and seeded either (1) directly onto a microporous membrane a...
Autores principales: | Leemans, Bart, Bromfield, Elizabeth G, Stout, Tom A E, Vos, Mabel, Van Der Ham, Hanna, Van Beek, Ramada, Van Soom, Ann, Gadella, Bart M, Henning, Heiko |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040661/ https://www.ncbi.nlm.nih.gov/pubmed/34962550 http://dx.doi.org/10.1093/biolre/ioab243 |
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