Two-color in vitro assay to visualize and quantify microtubule shaft dynamics

Microtubules are dynamic polymers where tubulin exchanges not only at the ends but also all along the microtubule shaft. In vitro reconstitutions are a vital approach to study microtubule tip dynamics, while direct observation of shaft dynamics is challenging. Here, we describe a dual-color in vitro...

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Detalles Bibliográficos
Autores principales: Andreu-Carbó, Mireia, Fernandes, Simon, Aumeier, Charlotte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043774/
https://www.ncbi.nlm.nih.gov/pubmed/35496777
http://dx.doi.org/10.1016/j.xpro.2022.101320

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043774/
https://www.ncbi.nlm.nih.gov/pubmed/35496777
http://dx.doi.org/10.1016/j.xpro.2022.101320

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