Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections

Photo-isolation chemistry (PIC) enables isolation of transcriptome information from locally defined areas by photo-irradiation. Here, we present an optimized PIC protocol for formalin-fixed frozen and paraffin mouse sections and fresh-frozen mouse sections. We describe tissue section preparation and...

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Detalles Bibliográficos
Autores principales: Honda, Mizuki, Kimura, Ryuichi, Harada, Akihito, Maehara, Kazumitsu, Tanaka, Kaori, Ohkawa, Yasuyuki, Oki, Shinya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046621/
https://www.ncbi.nlm.nih.gov/pubmed/35496796
http://dx.doi.org/10.1016/j.xpro.2022.101346

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046621/
https://www.ncbi.nlm.nih.gov/pubmed/35496796
http://dx.doi.org/10.1016/j.xpro.2022.101346

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