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Photo-isolation chemistry for high-resolution and deep spatial transcriptome with mouse tissue sections
Photo-isolation chemistry (PIC) enables isolation of transcriptome information from locally defined areas by photo-irradiation. Here, we present an optimized PIC protocol for formalin-fixed frozen and paraffin mouse sections and fresh-frozen mouse sections. We describe tissue section preparation and...
Autores principales: | Honda, Mizuki, Kimura, Ryuichi, Harada, Akihito, Maehara, Kazumitsu, Tanaka, Kaori, Ohkawa, Yasuyuki, Oki, Shinya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046621/ https://www.ncbi.nlm.nih.gov/pubmed/35496796 http://dx.doi.org/10.1016/j.xpro.2022.101346 |
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