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Systematic evaluation of TaqMan real-time polymerase chain reaction assays targeting the dsb and gltA loci of Ehrlichia canis in recombinant plasmids and naturally infected dogs

BACKGROUND AND AIM: Because of the diversity of local genotypes of Ehrlichia canis, genes targeted by TaqMan real-time polymerase chain reaction (RT-PCR) assays should be systematically evaluated. This study evaluated the amplification efficiency, linearity, precision, and sensitivity of two TaqMan...

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Detalles Bibliográficos
Autores principales: Sumpavong, Peeravit, Sricharern, Wanat, Inthong, Natnaree, Kaewmongkol, Gunn, Kaewmongkol, Sarawan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9047146/
https://www.ncbi.nlm.nih.gov/pubmed/35497947
http://dx.doi.org/10.14202/vetworld.2022.701-706