Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi

This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene containing the desired mutations that are resistant to Cas9-cleava...

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Detalles Bibliográficos
Autores principales: Picchi-Constante, Gisele Fernanda Assine, Hiraiwa, Priscila Mazzocchi, Marek, Martin, Rogerio, Vanessa Zulkievicz, Guerra-Slompo, Eloise Pavão, Romier, Christophe, Zanchin, Nilson Ivo Tonin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048117/
https://www.ncbi.nlm.nih.gov/pubmed/35496799
http://dx.doi.org/10.1016/j.xpro.2022.101324

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048117/
https://www.ncbi.nlm.nih.gov/pubmed/35496799
http://dx.doi.org/10.1016/j.xpro.2022.101324

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