Cargando…

Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi

This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene containing the desired mutations that are resistant to Cas9-cleava...

Descripción completa

Detalles Bibliográficos
Autores principales:	Picchi-Constante, Gisele Fernanda Assine, Hiraiwa, Priscila Mazzocchi, Marek, Martin, Rogerio, Vanessa Zulkievicz, Guerra-Slompo, Eloise Pavão, Romier, Christophe, Zanchin, Nilson Ivo Tonin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048117/ https://www.ncbi.nlm.nih.gov/pubmed/35496799 http://dx.doi.org/10.1016/j.xpro.2022.101324

Ejemplares similares

In cellulo and in vivo assays for compound testing against Trypanosoma cruzi
por: Guerra-Slompo, Eloise Pavão, et al.
Publicado: (2023)

Metacyclogenesis defects and gene expression hallmarks of histone deacetylase 4-deficient Trypanosoma cruzi cells
por: Picchi-Constante, Gisele Fernanda Assine, et al.
Publicado: (2021)

Dissecting Trypanosoma brucei RRP44 function in the maturation of segmented ribosomal RNA using a regulated genetic complementation system
por: Guerra-Slompo, Eloise Pavão, et al.
Publicado: (2023)

Trypanosoma brucei RRP44: a versatile enzyme for processing structured and non-structured RNA substrates
por: Cesaro, Giovanna, et al.
Publicado: (2022)

Knockout confirmation for Hurries: rapid genotype identification of Trypanosoma cruzi transfectants by polymerase chain reaction directly from liquid culture
por: Alcantara, Monica Visnieski, et al.
Publicado: (2014)

mRNA Localization Mechanisms in Trypanosoma cruzi
por: Alves, Lysangela R., et al.
Publicado: (2013)

A toolkit for recombinant production of seven human EGF family growth factors in active conformation
por: Ferreira, Arthur Schveitzer, et al.
Publicado: (2022)

Crystal structure of the Trypanosoma cruzi EIF4E5 translation factor homologue in complex with mRNA cap-4
por: Reolon, Lidia Watanabe, et al.
Publicado: (2019)

Performance Assessment of Four Chimeric Trypanosoma cruzi Antigens Based on Antigen-Antibody Detection for Diagnosis of Chronic Chagas Disease
por: Santos, Fred Luciano Neves, et al.
Publicado: (2016)

Detection of anti-Trypanosoma cruzi antibodies by chimeric antigens in chronic Chagas disease-individuals from endemic South American countries
por: Del-Rei, Rodrigo Pimenta, et al.
Publicado: (2019)

Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting
por: Dopico, Eva, et al.
Publicado: (2019)

Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi
por: Smircich, Pablo, et al.
Publicado: (2015)

Double-antigen sandwich ELISA based on chimeric antigens for detection of antibodies to Trypanosoma cruzi in human sera
por: Freitas, Natália Erdens Maron, et al.
Publicado: (2022)

Performance of Chimeric Trypanosoma cruzi Antigens in Serological Screening for Chagas Disease in Blood Banks
por: dos Santos, Emily Ferreira, et al.
Publicado: (2022)

Western blot using Trypanosoma cruzi chimeric recombinant proteins for the serodiagnosis of chronic Chagas disease: A proof-of-concept study
por: Daltro, Ramona Tavares, et al.
Publicado: (2022)

The Influence of Recombinational Processes to Induce Dormancy in Trypanosoma cruzi
por: Resende, Bruno Carvalho, et al.
Publicado: (2020)

Replication origin location might contribute to genetic variability in Trypanosoma cruzi
por: de Araujo, Christiane Bezerra, et al.
Publicado: (2020)

Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs
por: Leony, Leonardo M., et al.
Publicado: (2019)

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi
por: Peng, Duo, et al.
Publicado: (2014)

Cross-Reactivity Using Chimeric Trypanosoma cruzi Antigens: Diagnostic Performance in Settings Where Chagas Disease and American Cutaneous or Visceral Leishmaniasis Are Coendemic
por: Daltro, Ramona Tavares, et al.
Publicado: (2019)

Oral Transmission of Trypanosoma cruzi, Brazilian Amazon
por: Santana, Rosa Amélia G., et al.
Publicado: (2019)

Profiling the Trypanosoma cruzi Phosphoproteome
por: Marchini, Fabricio K., et al.
Publicado: (2011)

Clathrin expression in Trypanosoma cruzi
por: Kalb, Ligia Cristina, et al.
Publicado: (2014)

Endothelial Transmigration by Trypanosoma cruzi
por: Coates, Bria M., et al.
Publicado: (2013)

The N-myristoylome of Trypanosoma cruzi
por: Roberts, Adam J., et al.
Publicado: (2016)

Trypanosoma cruzi Gene Expression in Response to Gamma Radiation
por: Grynberg, Priscila, et al.
Publicado: (2012)

Phenothiazinium Dyes Are Active against Trypanosoma cruzi In Vitro
por: Bulhões Portapilla, Gisele, et al.
Publicado: (2019)

A CRISPR/Cas9-riboswitch-Based Method for Downregulation of Gene Expression in Trypanosoma cruzi
por: Lander, Noelia, et al.
Publicado: (2020)

Assessment of Cross-Reactivity of Chimeric Trypanosoma cruzi Antigens with Crithidia sp. LVH-60A: Implications for Accurate Diagnostics
por: Santos, Emily F., et al.
Publicado: (2023)

Metabolic Alteration of Trypanosoma cruzi during Differentiation of Epimastigote to Trypomastigote Forms
por: De-Simone, Salvatore G., et al.
Publicado: (2022)

Seroprevalence of Trypanosoma cruzi infection in blood donors in the extreme South of Brazil
por: Bianchi, Tanise Freitas, et al.
Publicado: (2022)

Proteomic Analysis of Trypanosoma cruzi Response to Ionizing Radiation Stress
por: Vieira, Helaine Graziele Santos, et al.
Publicado: (2014)

Specific inhibition of the differentiation of Trypanosoma cruzi
Publicado: (1984)

OBSERVATIONS ON THE RESPIRATION OF TRYPANOSOMA CRUZI IN CULTURE
por: von Brand, Theodor, et al.
Publicado: (1946)

Autochthonous Transmission of Trypanosoma cruzi, Louisiana
por: Dorn, Patricia L., et al.
Publicado: (2007)

Flagellar Motility of Trypanosoma cruzi Epimastigotes
por: Ballesteros-Rodea, G., et al.
Publicado: (2012)

Trypanosoma cruzi–Host Cell Interaction
por: De Souza, Wanderley
Publicado: (2014)

Immune Evasion Strategies of Trypanosoma cruzi
por: Flávia Nardy, Ana, et al.
Publicado: (2015)

Unveiling the Trypanosoma cruzi Nuclear Proteome
por: dos Santos Júnior, Agenor de Castro Moreira, et al.
Publicado: (2015)

Trypanosoma cruzi, organ transplant related
por: Latzke, Ana Belén, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_9km38kk4qsbfgpvcaj1tcjmmlf