Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media

We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compare...

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Detalles Bibliográficos
Autores principales: Kameda, Kotaro, Someya, Shota, Fujita, Jun, Fukuda, Keiichi, Tohyama, Shugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048135/
https://www.ncbi.nlm.nih.gov/pubmed/35496810
http://dx.doi.org/10.1016/j.xpro.2022.101341

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048135/
https://www.ncbi.nlm.nih.gov/pubmed/35496810
http://dx.doi.org/10.1016/j.xpro.2022.101341

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