Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media

We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compare...

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Detalles Bibliográficos
Autores principales: Kameda, Kotaro, Someya, Shota, Fujita, Jun, Fukuda, Keiichi, Tohyama, Shugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048135/
https://www.ncbi.nlm.nih.gov/pubmed/35496810
http://dx.doi.org/10.1016/j.xpro.2022.101341
Descripción
Sumario:We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to Someya et al. (2021).

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