Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media

We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compare...

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Detalles Bibliográficos
Autores principales: Kameda, Kotaro, Someya, Shota, Fujita, Jun, Fukuda, Keiichi, Tohyama, Shugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048135/
https://www.ncbi.nlm.nih.gov/pubmed/35496810
http://dx.doi.org/10.1016/j.xpro.2022.101341
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author Kameda, Kotaro
Someya, Shota
Fujita, Jun
Fukuda, Keiichi
Tohyama, Shugo
author_facet Kameda, Kotaro
Someya, Shota
Fujita, Jun
Fukuda, Keiichi
Tohyama, Shugo
author_sort Kameda, Kotaro
collection PubMed
description We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to Someya et al. (2021).
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spelling pubmed-90481352022-04-29 Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media Kameda, Kotaro Someya, Shota Fujita, Jun Fukuda, Keiichi Tohyama, Shugo STAR Protoc Protocol We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compared to using a conventional medium alone. Here, we describe the steps for enhanced proliferation of hPSCs from dermal fibroblasts or peripheral blood cells, but the protocol can be applied to any hPSCs. For complete details on the use and execution of this protocol, please refer to Someya et al. (2021). Elsevier 2022-04-22 /pmc/articles/PMC9048135/ /pubmed/35496810 http://dx.doi.org/10.1016/j.xpro.2022.101341 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Kameda, Kotaro
Someya, Shota
Fujita, Jun
Fukuda, Keiichi
Tohyama, Shugo
Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
title Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
title_full Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
title_fullStr Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
title_full_unstemmed Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
title_short Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
title_sort protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048135/
https://www.ncbi.nlm.nih.gov/pubmed/35496810
http://dx.doi.org/10.1016/j.xpro.2022.101341
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