Cargando…

Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media

We describe a protocol for the efficient culture of human pluripotent stem cells (hPSCs) by supplementing conventional culture medium with L-tryptophan (TRP). TRP is an essential amino acid that is widely available at an affordable cost, thereby allowing cost-effective proliferation of hPSCs compare...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kameda, Kotaro, Someya, Shota, Fujita, Jun, Fukuda, Keiichi, Tohyama, Shugo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9048135/ https://www.ncbi.nlm.nih.gov/pubmed/35496810 http://dx.doi.org/10.1016/j.xpro.2022.101341

Ejemplares similares

Manipulation of Pluripotent Stem Cell Metabolism for Clinical Application
por: Tohyama, Shugo, et al.
Publicado: (2017)

Tryptophan Metabolism Regulates Proliferative Capacity of Human Pluripotent Stem Cells
por: Someya, Shota, et al.
Publicado: (2021)

Toward the realization of cardiac regenerative medicine using pluripotent stem cells
por: Kishino, Yoshikazu, et al.
Publicado: (2020)

Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis
por: Tanosaki, Sho, et al.
Publicado: (2022)

Metabolism of human pluripotent stem cells and differentiated cells for regenerative therapy: a focus on cardiomyocytes
por: Tanosaki, Sho, et al.
Publicado: (2021)

Scalable manufacturing of clinical‐grade differentiated cardiomyocytes derived from human‐induced pluripotent stem cells for regenerative therapy
por: Morita, Yuika, et al.
Publicado: (2022)

An effective detachment system for human induced pluripotent stem cells cultured on multilayered cultivation substrates using resonance vibrations
por: Terao, Yusuke, et al.
Publicado: (2019)

The Present State and Future Perspectives of Cardiac Regenerative Therapy Using Human Pluripotent Stem Cells
por: Soma, Yusuke, et al.
Publicado: (2021)

Fatty Acid Synthesis Is Indispensable for Survival of Human Pluripotent Stem Cells
por: Tanosaki, Sho, et al.
Publicado: (2020)

Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes
por: Tohyama, Shugo, et al.
Publicado: (2017)

Optimized protocol for analysis of neural stem proliferation in human-pluripotent-stem-cell-derived cerebral organoids
por: Tang, Xiao-Yan, et al.
Publicado: (2023)

Derivation of Transgene-Free Human Induced Pluripotent Stem Cells from Human Peripheral T Cells in Defined Culture Conditions
por: Kishino, Yoshikazu, et al.
Publicado: (2014)

Metabolic Regulation of Cardiac Differentiation and Maturation in Pluripotent Stem Cells: A Lesson from Heart Development
por: Morita, Yuika, et al.
Publicado: (2020)

Protocol for germ lineage differentiation of primed human pluripotent stem cells using chemically defined, nutrient-balanced media
por: Lu, Vivian, et al.
Publicado: (2022)

Scaffold-Free Tubular Engineered Heart Tissue From Human Induced Pluripotent Stem Cells Using Bio-3D Printing Technology in vivo
por: Kawai, Yujiro, et al.
Publicado: (2022)

Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media
por: Astro, Veronica, et al.
Publicado: (2023)

Differentiation of functional astrocytes from human-induced pluripotent stem cells in chemically defined media
por: Perriot, Sylvain, et al.
Publicado: (2021)

Seamless and non-destructive monitoring of extracellular microRNAs during cardiac differentiation from human pluripotent stem cells
por: Sekine, Otoya, et al.
Publicado: (2023)

Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells
por: Bejoy, Julie, et al.
Publicado: (2021)

Pluripotent Stem Cell-Derived Hepatocytes Inhibit T Cell Proliferation In Vitro through Tryptophan Starvation
por: Romano, Marco, et al.
Publicado: (2021)

Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells
por: Wei, Yanxing, et al.
Publicado: (2022)

Generation and Characterization of Functional Cardiomyocytes Derived from Human T Cell-Derived Induced Pluripotent Stem Cells
por: Seki, Tomohisa, et al.
Publicado: (2014)

Optimized protocol for naive human pluripotent stem cell-derived trophoblast induction
por: Io, Shingo, et al.
Publicado: (2021)

Protocol for the Generation of Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
por: Nafria, Monica, et al.
Publicado: (2020)

A protocol for rapid pericyte differentiation of human induced pluripotent stem cells
por: Aisenbrey, Elizabeth A., et al.
Publicado: (2021)

Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
por: Mucci, Sofía, et al.
Publicado: (2022)

Protocol to generate cardiac pericytes from human induced pluripotent stem cells
por: Shen, Mengcheng, et al.
Publicado: (2023)

Protocol for the design, conduct, and evaluation of prime editing in human pluripotent stem cells
por: Wu, Youjun, et al.
Publicado: (2023)

Optimized protocol to derive germline stem-cell-like cells from mouse pluripotent stem cells
por: Ishikura, Yukiko, et al.
Publicado: (2022)

Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells
por: Li, Ronghui, et al.
Publicado: (2021)

Protocol for Large-Scale Production of Kidney Organoids from Human Pluripotent Stem Cells
por: Sander, Veronika, et al.
Publicado: (2020)

A protocol to differentiate nociceptors, mechanoreceptors, and proprioceptors from human pluripotent stem cells
por: Saito-Diaz, Kenyi, et al.
Publicado: (2022)

Protocol for selecting single human pluripotent stem cells using a modified micropipetter
por: Mojica-Perez, Sandra P., et al.
Publicado: (2023)

Protocol to differentiate monolayer human induced pluripotent stem cells into inflammatory responsive astrocytes
por: Giordano, Anna Maria Sole, et al.
Publicado: (2023)

An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells
por: Haque, Mohammad, et al.
Publicado: (2021)

Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
por: Yin, Xiling, et al.
Publicado: (2021)

An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells
por: Lyra-Leite, Davi Marco, et al.
Publicado: (2021)

A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes
por: Knight, Walter E., et al.
Publicado: (2021)

Optimized protocol for the generation of functional human induced-pluripotent-stem-cell-derived dopaminergic neurons
por: Sheta, Razan, et al.
Publicado: (2023)

Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell-derived cardiomyocytes
por: Zhang, Joe Z., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_1dol4jgugo1s7rap16djgk3jng