Stable immobilization of aldehyde ketone reductase mutants containing nonstandard amino acids on an epoxy resin via strain-promoted alkyne–azide cycloaddition

To avoid random chemical linkage and achieve precisely directed immobilization, mutant enzymes were obtained and immobilized using an incorporated reactive nonstandard amino acid (NSAA). For this purpose, aldehyde ketone reductase (AKR) was used as a model enzyme, and 110Y, 114Y, 143Y, 162Q and 189Q...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Huimin, Yin, Youcheng, Wang, Anming, Li, Ningning, Wang, Ru, Zhang, Jing, Chen, Xinxin, Pei, Xiaolin, Xie, Tian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049136/
https://www.ncbi.nlm.nih.gov/pubmed/35496112
http://dx.doi.org/10.1039/c9ra09067c

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9049136/
https://www.ncbi.nlm.nih.gov/pubmed/35496112
http://dx.doi.org/10.1039/c9ra09067c

Ejemplares similares