Cargando…

Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme

Carbon quantum dots (CQDs) were synthesized from litchi peel, exhibiting a peroxidase-like activity and enabling the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in association with H(2)O(2) to generate blue oxidized TMB (ox-TMB) with a strong absorption peak at 652 nm. Interestingly, the ox-TM...

Descripción completa

Detalles Bibliográficos
Autores principales: Shu, Xin, Chang, Yuwai, Wen, Huizhong, Yao, Xiaotiao, Wang, Yilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052118/
https://www.ncbi.nlm.nih.gov/pubmed/35497159
http://dx.doi.org/10.1039/d0ra02105a
_version_ 1784696716256083968
author Shu, Xin
Chang, Yuwai
Wen, Huizhong
Yao, Xiaotiao
Wang, Yilin
author_facet Shu, Xin
Chang, Yuwai
Wen, Huizhong
Yao, Xiaotiao
Wang, Yilin
author_sort Shu, Xin
collection PubMed
description Carbon quantum dots (CQDs) were synthesized from litchi peel, exhibiting a peroxidase-like activity and enabling the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in association with H(2)O(2) to generate blue oxidized TMB (ox-TMB) with a strong absorption peak at 652 nm. Interestingly, the ox-TMB could be further reduced by ascorbic acid (AA) leading to fading of the blue color and an absorbance decrease. Thus, a convenient and sensitive colorimetric method for detection of AA using CQDs as peroxidase mimics was established. Several factors, such as acidity, temperature, incubating time, and TMB concentration, which might influence the response of the analysis signal, were optimized. The results showed that the decrease of absorbance (ΔA) was in good linear agreement with AA concentration in the range of 1.0–105 μM, with a low detection limit of 0.14 μM. The feasibility of this method was also investigated in commercial beverages with the 94.3–110.0% recovery.
format Online
Article
Text
id pubmed-9052118
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90521182022-04-29 Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme Shu, Xin Chang, Yuwai Wen, Huizhong Yao, Xiaotiao Wang, Yilin RSC Adv Chemistry Carbon quantum dots (CQDs) were synthesized from litchi peel, exhibiting a peroxidase-like activity and enabling the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in association with H(2)O(2) to generate blue oxidized TMB (ox-TMB) with a strong absorption peak at 652 nm. Interestingly, the ox-TMB could be further reduced by ascorbic acid (AA) leading to fading of the blue color and an absorbance decrease. Thus, a convenient and sensitive colorimetric method for detection of AA using CQDs as peroxidase mimics was established. Several factors, such as acidity, temperature, incubating time, and TMB concentration, which might influence the response of the analysis signal, were optimized. The results showed that the decrease of absorbance (ΔA) was in good linear agreement with AA concentration in the range of 1.0–105 μM, with a low detection limit of 0.14 μM. The feasibility of this method was also investigated in commercial beverages with the 94.3–110.0% recovery. The Royal Society of Chemistry 2020-04-16 /pmc/articles/PMC9052118/ /pubmed/35497159 http://dx.doi.org/10.1039/d0ra02105a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Shu, Xin
Chang, Yuwai
Wen, Huizhong
Yao, Xiaotiao
Wang, Yilin
Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme
title Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme
title_full Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme
title_fullStr Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme
title_full_unstemmed Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme
title_short Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme
title_sort colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052118/
https://www.ncbi.nlm.nih.gov/pubmed/35497159
http://dx.doi.org/10.1039/d0ra02105a
work_keys_str_mv AT shuxin colorimetricdeterminationofascorbicacidbasedoncarbonquantumdotsasperoxidasemimeticenzyme
AT changyuwai colorimetricdeterminationofascorbicacidbasedoncarbonquantumdotsasperoxidasemimeticenzyme
AT wenhuizhong colorimetricdeterminationofascorbicacidbasedoncarbonquantumdotsasperoxidasemimeticenzyme
AT yaoxiaotiao colorimetricdeterminationofascorbicacidbasedoncarbonquantumdotsasperoxidasemimeticenzyme
AT wangyilin colorimetricdeterminationofascorbicacidbasedoncarbonquantumdotsasperoxidasemimeticenzyme