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Active secretion of a thermostable transglutaminase variant in Escherichia coli

BACKGROUND: Streptomyces mobaraenesis transglutaminase (smTG) is widely used to generate protein crosslinking or attachment of small molecules. However, the low thermostability is a main obstacle for smTG application. In addition, it is still hard to achieve the secretory expression of active smTG i...

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Detalles Bibliográficos
Autores principales: Wang, Xinglong, Zhao, Beichen, Du, Jianhui, Xu, Yameng, Zhu, Xuewen, Zhou, Jingwen, Rao, Shengqi, Du, Guocheng, Chen, Jian, Liu, Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052465/
https://www.ncbi.nlm.nih.gov/pubmed/35488338
http://dx.doi.org/10.1186/s12934-022-01801-9