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Semi-nested RT-PCR enables sensitive and high-throughput detection of SARS-CoV-2 based on melting analysis

BACKGROUND: Asymptomatic transmission was found to be the Achilles’ heel of the symptom-based screening strategy, necessitating the implementation of mass testing to efficiently contain the transmission of COVID-19 pandemic. However, the global shortage of molecular reagents and the low throughput o...

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Detalles Bibliográficos
Autores principales: Thi Nguyen, Ngoc Anh, Thi Bui, Hoai, Thi-Huong Pham, Quynh, Thi Thao Hoang, Ly, Xuan Ta, Hung, Heikkinen, Timo, Van Le, Duyet, Dinh Van, Trang, Quoc Ngo, Nam, Thi Hong Huynh, Phuong, Thi Huyen Tran, Trang, Quoc Phan, Hoan, Van Hoang, Luong, van Doorn, H.Rogier, Thi Ngoc Nguyen, Diep, Thi Nguyen, Tam, Sy Vo, Nam, Viet Vo, Cuong, Khac Trinh, Sau, The Pham, Tai, Duc Le, Quang, Van Le, Phan, Thai Nguyen, Son, Thi Tran, Loan, Dinh Vu, Toan, Vu Nguyen, Quynh Anh, Thi Trieu, Nguyet, Thi Le, Thuy, Dinh Nguyen, Ung, Steman, Jakob, Huu Ho, Tho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9052777/
https://www.ncbi.nlm.nih.gov/pubmed/35500877
http://dx.doi.org/10.1016/j.cca.2022.04.997