Induction of ADCC by a folic acid–mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells

We developed conjugates between monoclonal antibody (mAb) and folic acid (FA) by using a tryptophan (Trp)-selective reaction, which yields relatively homogenous products compared to conventional methods. The obtained mAb–FA conjugates showed significant cellular cytotoxicity toward folate receptor-e...

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Autores principales: Tagawa, Hiroshi, Maruyama, Katsuya, Sasaki, Koichi, Konoue, Natsuki, Kishimura, Akihiro, Kanai, Motomu, Mori, Takeshi, Oisaki, Kounosuke, Katayama, Yoshiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9053046/
https://www.ncbi.nlm.nih.gov/pubmed/35498849
http://dx.doi.org/10.1039/d0ra03291c
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author Tagawa, Hiroshi
Maruyama, Katsuya
Sasaki, Koichi
Konoue, Natsuki
Kishimura, Akihiro
Kanai, Motomu
Mori, Takeshi
Oisaki, Kounosuke
Katayama, Yoshiki
author_facet Tagawa, Hiroshi
Maruyama, Katsuya
Sasaki, Koichi
Konoue, Natsuki
Kishimura, Akihiro
Kanai, Motomu
Mori, Takeshi
Oisaki, Kounosuke
Katayama, Yoshiki
author_sort Tagawa, Hiroshi
collection PubMed
description We developed conjugates between monoclonal antibody (mAb) and folic acid (FA) by using a tryptophan (Trp)-selective reaction, which yields relatively homogenous products compared to conventional methods. The obtained mAb–FA conjugates showed significant cellular cytotoxicity toward folate receptor-expressing cancer cells, demonstrating that the conjugates retained the Fc region's original function.
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spelling pubmed-90530462022-04-29 Induction of ADCC by a folic acid–mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells Tagawa, Hiroshi Maruyama, Katsuya Sasaki, Koichi Konoue, Natsuki Kishimura, Akihiro Kanai, Motomu Mori, Takeshi Oisaki, Kounosuke Katayama, Yoshiki RSC Adv Chemistry We developed conjugates between monoclonal antibody (mAb) and folic acid (FA) by using a tryptophan (Trp)-selective reaction, which yields relatively homogenous products compared to conventional methods. The obtained mAb–FA conjugates showed significant cellular cytotoxicity toward folate receptor-expressing cancer cells, demonstrating that the conjugates retained the Fc region's original function. The Royal Society of Chemistry 2020-04-29 /pmc/articles/PMC9053046/ /pubmed/35498849 http://dx.doi.org/10.1039/d0ra03291c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Tagawa, Hiroshi
Maruyama, Katsuya
Sasaki, Koichi
Konoue, Natsuki
Kishimura, Akihiro
Kanai, Motomu
Mori, Takeshi
Oisaki, Kounosuke
Katayama, Yoshiki
Induction of ADCC by a folic acid–mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells
title Induction of ADCC by a folic acid–mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells
title_full Induction of ADCC by a folic acid–mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells
title_fullStr Induction of ADCC by a folic acid–mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells
title_full_unstemmed Induction of ADCC by a folic acid–mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells
title_short Induction of ADCC by a folic acid–mAb conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells
title_sort induction of adcc by a folic acid–mab conjugate prepared by tryptophan-selective reaction toward folate-receptor-positive cancer cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9053046/
https://www.ncbi.nlm.nih.gov/pubmed/35498849
http://dx.doi.org/10.1039/d0ra03291c
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