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Nanocomposite liposomes for pH-controlled porphyrin release into human prostate cancer cells
It is both challenging and desirable to have drug sensitizers released at acidic tumor pH for photodynamic therapy in cancer treatment. A pH-responsive carrier was prepared, in which fumed silica-attached 5,10,15,20-tetrakis(4-trimethylammoniophenyl)porphyrin (TTMAPP) was encapsulated into 1,2-diole...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9053171/ https://www.ncbi.nlm.nih.gov/pubmed/35496928 http://dx.doi.org/10.1039/d0ra00846j |
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author | Fuentes, German V. Doucet, Eric N. Abraham, Alyson Rodgers, Nikki K. Alonso, Felix Euceda, Nelson Quinones, Michael H. Riascos, Penelope A. Pierre, Kristelle Sarker, Nuhash H. Dhar-Mascareno, Manya Cotlet, Mircea Kisslinger, Kim Camino, Fernando Li, Mingxing Lu, Fang Gao, Ruomei |
author_facet | Fuentes, German V. Doucet, Eric N. Abraham, Alyson Rodgers, Nikki K. Alonso, Felix Euceda, Nelson Quinones, Michael H. Riascos, Penelope A. Pierre, Kristelle Sarker, Nuhash H. Dhar-Mascareno, Manya Cotlet, Mircea Kisslinger, Kim Camino, Fernando Li, Mingxing Lu, Fang Gao, Ruomei |
author_sort | Fuentes, German V. |
collection | PubMed |
description | It is both challenging and desirable to have drug sensitizers released at acidic tumor pH for photodynamic therapy in cancer treatment. A pH-responsive carrier was prepared, in which fumed silica-attached 5,10,15,20-tetrakis(4-trimethylammoniophenyl)porphyrin (TTMAPP) was encapsulated into 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) nanocomposite liposomes. The sizes of agglomerates were determined by dynamic light scattering to be 115 nm for silica and 295 nm for silica-TTMAPP-DOPC liposomes. Morphological changes were also found in TEM images, showing liposome formation at pH 8.5 but collapse upon silanol protonation. TTMAPP release is enhanced from 13% at pH 7.5 to 80% at pH 2.3, as determined spectrophotometrically through dialysis membranes. Fluorescence emission of TTMAPP encapsulated in the dry film of liposomes was reduced to half at pH 8.6 when compared to that at pH 5.4, while the production of singlet oxygen was quintupled at pH 5.0 compared to pH 7.6. Upon treatment of human prostate cancer cells with liposomes containing 6.7 μM, 13 μM, 17 μM and 20 μM TTMAPP, the cell viabilities were determined to be 60%, 18%, 20% and 5% at pH 5.4; 58%, 30%, 25% and 10% at pH 6.3; and 90%, 82%, 68% and 35% at pH 7.4, respectively. Light-induced apoptosis in cancerous cells was only observed in the presence of liposomes at pH 6.3 and pH 5.4 but not at pH 7.4, as indicated by chromatin condensation. |
format | Online Article Text |
id | pubmed-9053171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90531712022-04-29 Nanocomposite liposomes for pH-controlled porphyrin release into human prostate cancer cells Fuentes, German V. Doucet, Eric N. Abraham, Alyson Rodgers, Nikki K. Alonso, Felix Euceda, Nelson Quinones, Michael H. Riascos, Penelope A. Pierre, Kristelle Sarker, Nuhash H. Dhar-Mascareno, Manya Cotlet, Mircea Kisslinger, Kim Camino, Fernando Li, Mingxing Lu, Fang Gao, Ruomei RSC Adv Chemistry It is both challenging and desirable to have drug sensitizers released at acidic tumor pH for photodynamic therapy in cancer treatment. A pH-responsive carrier was prepared, in which fumed silica-attached 5,10,15,20-tetrakis(4-trimethylammoniophenyl)porphyrin (TTMAPP) was encapsulated into 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) nanocomposite liposomes. The sizes of agglomerates were determined by dynamic light scattering to be 115 nm for silica and 295 nm for silica-TTMAPP-DOPC liposomes. Morphological changes were also found in TEM images, showing liposome formation at pH 8.5 but collapse upon silanol protonation. TTMAPP release is enhanced from 13% at pH 7.5 to 80% at pH 2.3, as determined spectrophotometrically through dialysis membranes. Fluorescence emission of TTMAPP encapsulated in the dry film of liposomes was reduced to half at pH 8.6 when compared to that at pH 5.4, while the production of singlet oxygen was quintupled at pH 5.0 compared to pH 7.6. Upon treatment of human prostate cancer cells with liposomes containing 6.7 μM, 13 μM, 17 μM and 20 μM TTMAPP, the cell viabilities were determined to be 60%, 18%, 20% and 5% at pH 5.4; 58%, 30%, 25% and 10% at pH 6.3; and 90%, 82%, 68% and 35% at pH 7.4, respectively. Light-induced apoptosis in cancerous cells was only observed in the presence of liposomes at pH 6.3 and pH 5.4 but not at pH 7.4, as indicated by chromatin condensation. The Royal Society of Chemistry 2020-05-01 /pmc/articles/PMC9053171/ /pubmed/35496928 http://dx.doi.org/10.1039/d0ra00846j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Fuentes, German V. Doucet, Eric N. Abraham, Alyson Rodgers, Nikki K. Alonso, Felix Euceda, Nelson Quinones, Michael H. Riascos, Penelope A. Pierre, Kristelle Sarker, Nuhash H. Dhar-Mascareno, Manya Cotlet, Mircea Kisslinger, Kim Camino, Fernando Li, Mingxing Lu, Fang Gao, Ruomei Nanocomposite liposomes for pH-controlled porphyrin release into human prostate cancer cells |
title | Nanocomposite liposomes for pH-controlled porphyrin release into human prostate cancer cells |
title_full | Nanocomposite liposomes for pH-controlled porphyrin release into human prostate cancer cells |
title_fullStr | Nanocomposite liposomes for pH-controlled porphyrin release into human prostate cancer cells |
title_full_unstemmed | Nanocomposite liposomes for pH-controlled porphyrin release into human prostate cancer cells |
title_short | Nanocomposite liposomes for pH-controlled porphyrin release into human prostate cancer cells |
title_sort | nanocomposite liposomes for ph-controlled porphyrin release into human prostate cancer cells |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9053171/ https://www.ncbi.nlm.nih.gov/pubmed/35496928 http://dx.doi.org/10.1039/d0ra00846j |
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