A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of...

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Detalles Bibliográficos
Autores principales: Popescu, Codruţa C., Stoian, Marius C., Cucos, Lia-Maria, Coman, Anca G., Radoi, Antonio, Paun, Anca, Hădade, Niculina D., Gautier, Arnaud, Popescu, Costin-Ioan, Matache, Mihaela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055129/
https://www.ncbi.nlm.nih.gov/pubmed/35517349
http://dx.doi.org/10.1039/d0ra02382e

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055129/
https://www.ncbi.nlm.nih.gov/pubmed/35517349
http://dx.doi.org/10.1039/d0ra02382e

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