A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems

We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of...

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Detalles Bibliográficos
Autores principales: Popescu, Codruţa C., Stoian, Marius C., Cucos, Lia-Maria, Coman, Anca G., Radoi, Antonio, Paun, Anca, Hădade, Niculina D., Gautier, Arnaud, Popescu, Costin-Ioan, Matache, Mihaela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055129/
https://www.ncbi.nlm.nih.gov/pubmed/35517349
http://dx.doi.org/10.1039/d0ra02382e
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author Popescu, Codruţa C.
Stoian, Marius C.
Cucos, Lia-Maria
Coman, Anca G.
Radoi, Antonio
Paun, Anca
Hădade, Niculina D.
Gautier, Arnaud
Popescu, Costin-Ioan
Matache, Mihaela
author_facet Popescu, Codruţa C.
Stoian, Marius C.
Cucos, Lia-Maria
Coman, Anca G.
Radoi, Antonio
Paun, Anca
Hădade, Niculina D.
Gautier, Arnaud
Popescu, Costin-Ioan
Matache, Mihaela
author_sort Popescu, Codruţa C.
collection PubMed
description We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.
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spelling pubmed-90551292022-05-04 A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems Popescu, Codruţa C. Stoian, Marius C. Cucos, Lia-Maria Coman, Anca G. Radoi, Antonio Paun, Anca Hădade, Niculina D. Gautier, Arnaud Popescu, Costin-Ioan Matache, Mihaela RSC Adv Chemistry We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells. The Royal Society of Chemistry 2020-06-23 /pmc/articles/PMC9055129/ /pubmed/35517349 http://dx.doi.org/10.1039/d0ra02382e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Popescu, Codruţa C.
Stoian, Marius C.
Cucos, Lia-Maria
Coman, Anca G.
Radoi, Antonio
Paun, Anca
Hădade, Niculina D.
Gautier, Arnaud
Popescu, Costin-Ioan
Matache, Mihaela
A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems
title A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems
title_full A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems
title_fullStr A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems
title_full_unstemmed A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems
title_short A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems
title_sort polycarboxylic chelating ligand for efficient resin purification of his-tagged proteins expressed in mammalian systems
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055129/
https://www.ncbi.nlm.nih.gov/pubmed/35517349
http://dx.doi.org/10.1039/d0ra02382e
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