Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA

Sch-642305 is an unusual bicyclic 10-membered macrolide produced by the filamentous fungus Phomopsis sp. CMU-LMA for which no biosynthetic evidence exists. Here, we generate a draft genome sequence of the producing organism and discover the biosynthetic gene cluster responsible for formation of Sch-...

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Autores principales: Trenti, Francesco, Lebe, Karen E., Adelin, Emilie, Ouazzani, Jamal, Schotte, Carsten, Cox, Russell J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055631/
https://www.ncbi.nlm.nih.gov/pubmed/35516937
http://dx.doi.org/10.1039/d0ra05311b
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author Trenti, Francesco
Lebe, Karen E.
Adelin, Emilie
Ouazzani, Jamal
Schotte, Carsten
Cox, Russell J.
author_facet Trenti, Francesco
Lebe, Karen E.
Adelin, Emilie
Ouazzani, Jamal
Schotte, Carsten
Cox, Russell J.
author_sort Trenti, Francesco
collection PubMed
description Sch-642305 is an unusual bicyclic 10-membered macrolide produced by the filamentous fungus Phomopsis sp. CMU-LMA for which no biosynthetic evidence exists. Here, we generate a draft genome sequence of the producing organism and discover the biosynthetic gene cluster responsible for formation of Sch-642305. Targeted gene disruptions together with reconstitution of the pathway in the heterologous host Aspergillus oryzae dissect key chemical steps and shed light on a series of oxidoreductions occuring in the pathway.
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spelling pubmed-90556312022-05-04 Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA Trenti, Francesco Lebe, Karen E. Adelin, Emilie Ouazzani, Jamal Schotte, Carsten Cox, Russell J. RSC Adv Chemistry Sch-642305 is an unusual bicyclic 10-membered macrolide produced by the filamentous fungus Phomopsis sp. CMU-LMA for which no biosynthetic evidence exists. Here, we generate a draft genome sequence of the producing organism and discover the biosynthetic gene cluster responsible for formation of Sch-642305. Targeted gene disruptions together with reconstitution of the pathway in the heterologous host Aspergillus oryzae dissect key chemical steps and shed light on a series of oxidoreductions occuring in the pathway. The Royal Society of Chemistry 2020-07-21 /pmc/articles/PMC9055631/ /pubmed/35516937 http://dx.doi.org/10.1039/d0ra05311b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Trenti, Francesco
Lebe, Karen E.
Adelin, Emilie
Ouazzani, Jamal
Schotte, Carsten
Cox, Russell J.
Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA
title Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA
title_full Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA
title_fullStr Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA
title_full_unstemmed Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA
title_short Investigating the biosynthesis of Sch-642305 in the fungus Phomopsis sp. CMU-LMA
title_sort investigating the biosynthesis of sch-642305 in the fungus phomopsis sp. cmu-lma
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055631/
https://www.ncbi.nlm.nih.gov/pubmed/35516937
http://dx.doi.org/10.1039/d0ra05311b
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