A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly

We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR–Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR–Cas9, the detection method showed a s...

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Detalles Bibliográficos
Autores principales: Liu, Yang, Li, Shihong, Zhang, Likun, Zhao, Qian, Li, Nuo, Wu, Yuxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055656/
https://www.ncbi.nlm.nih.gov/pubmed/35519131
http://dx.doi.org/10.1039/d0ra03603j

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055656/
https://www.ncbi.nlm.nih.gov/pubmed/35519131
http://dx.doi.org/10.1039/d0ra03603j

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