A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly

We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR–Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR–Cas9, the detection method showed a s...

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Detalles Bibliográficos
Autores principales: Liu, Yang, Li, Shihong, Zhang, Likun, Zhao, Qian, Li, Nuo, Wu, Yuxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055656/
https://www.ncbi.nlm.nih.gov/pubmed/35519131
http://dx.doi.org/10.1039/d0ra03603j
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author Liu, Yang
Li, Shihong
Zhang, Likun
Zhao, Qian
Li, Nuo
Wu, Yuxin
author_facet Liu, Yang
Li, Shihong
Zhang, Likun
Zhao, Qian
Li, Nuo
Wu, Yuxin
author_sort Liu, Yang
collection PubMed
description We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR–Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR–Cas9, the detection method showed a sensitivity of 23.5 fM and was applied for accurate exosomal miR-21 detection and in situ miR-21 imaging.
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spelling pubmed-90556562022-05-04 A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly Liu, Yang Li, Shihong Zhang, Likun Zhao, Qian Li, Nuo Wu, Yuxin RSC Adv Chemistry We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR–Cas9 and catalytic hairpin assembly. With the cascade signal amplification of catalytic hairpin assembly and high specific recognition of CRISPR–Cas9, the detection method showed a sensitivity of 23.5 fM and was applied for accurate exosomal miR-21 detection and in situ miR-21 imaging. The Royal Society of Chemistry 2020-07-28 /pmc/articles/PMC9055656/ /pubmed/35519131 http://dx.doi.org/10.1039/d0ra03603j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Liu, Yang
Li, Shihong
Zhang, Likun
Zhao, Qian
Li, Nuo
Wu, Yuxin
A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly
title A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly
title_full A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly
title_fullStr A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly
title_full_unstemmed A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly
title_short A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly
title_sort sensitive and specific method for microrna detection and in situ imaging based on a crispr–cas9 modified catalytic hairpin assembly
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055656/
https://www.ncbi.nlm.nih.gov/pubmed/35519131
http://dx.doi.org/10.1039/d0ra03603j
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