The selection of highly specific and selective aptamers using modified SELEX and their use in process analytical techniques for Lucentis bioproduction

Aptamers for Lucentis were selected using 10 rounds of a modified and highly stringent SELEX process. Affinity column chromatography was used for the binding, partitioning, and elution steps, and the regeneration of ssDNA was performed via asymmetric PCR in the SELEX process. The interaction of apta...

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Detalles Bibliográficos
Autores principales: Bhardwaj, Tanu, Rathore, Anurag S., Jha, Sandeep Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055848/
https://www.ncbi.nlm.nih.gov/pubmed/35520059
http://dx.doi.org/10.1039/d0ra03542d

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9055848/
https://www.ncbi.nlm.nih.gov/pubmed/35520059
http://dx.doi.org/10.1039/d0ra03542d

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