Single-sister labeling in the C. elegans germline using the nucleotide analog EdU

Reciprocal exchanges between genetically identical sister chromatids (sister chromatid exchanges or SCEs) have been challenging to study. Here, we describe a protocol that utilizes a pulse/chase of the thymidine analog 5-ethyl-3′-deoxyuridine (EdU) in combination with click chemistry and antibody la...

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Detalles Bibliográficos
Autores principales: Almanzar, David E., Hamrick, Antonia, Rog, Ofer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059099/
https://www.ncbi.nlm.nih.gov/pubmed/35509971
http://dx.doi.org/10.1016/j.xpro.2022.101344

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9059099/
https://www.ncbi.nlm.nih.gov/pubmed/35509971
http://dx.doi.org/10.1016/j.xpro.2022.101344

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