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Photopharmacological modulation of native CRAC channels using azoboronate photoswitches

Store-operated calcium entry through calcium release–activated calcium (CRAC) channels replenishes intracellular calcium stores and plays a critical role in cellular calcium signaling. CRAC channels are activated by tightly regulated interaction between the endoplasmic reticulum (ER) calcium sensor...

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Detalles Bibliográficos
Autores principales: Udasin, Ronald, Sil, Anwesha, Zomot, Elia, Cohen, Hadas Achildiev, Haj, Jozafina, Engelmayer, Nurit, Lev, Shaya, Binshtok, Alexander M., Shaked, Yuval, Kienzler, Michael A., Palty, Raz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060504/
https://www.ncbi.nlm.nih.gov/pubmed/35312368
http://dx.doi.org/10.1073/pnas.2118160119
Descripción
Sumario:Store-operated calcium entry through calcium release–activated calcium (CRAC) channels replenishes intracellular calcium stores and plays a critical role in cellular calcium signaling. CRAC channels are activated by tightly regulated interaction between the endoplasmic reticulum (ER) calcium sensor STIM proteins and plasma membrane (PM) Orai channels. Our current understanding of the role of STIM–Orai-dependent calcium signals under physiologically relevant conditions remains limited in part due to a lack of spatiotemporally precise methods for direct manipulation of endogenous CRAC channels. Here, we report the synthesis and characterization of azoboronate light-operated CRAC channel inhibitors (LOCIs) that allow for a dynamic and fully reversible remote modulation of the function of native CRAC channels using ultraviolet (UV) and visible light. We demonstrate the use of LOCI-1 to modulate gene expression in T lymphocytes, cancer cell seeding at metastatic sites, and pain-related behavior.