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High-throughput screening of high lactic acid-producing Bacillus coagulans by droplet microfluidic based flow cytometry with fluorescence activated cell sorting

A high-throughput screening system based on droplet microfluidic sorting was developed and employed for screening of high lactic acid-producing Bacillus coagulans. In this system, water-in-oil-in-water (W/O/W) droplets, which were ∼12 pL in volume were used as picoliter-reactors for lactic acid ferm...

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Detalles Bibliográficos
Autores principales: Zhu, Xu-Dong, Shi, Xiang, Wang, Shu-Wen, Chu, Ju, Zhu, Wei-Hong, Ye, Bang-Ce, Zuo, Peng, Wang, Yong-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9060631/
https://www.ncbi.nlm.nih.gov/pubmed/35520173
http://dx.doi.org/10.1039/c8ra09684h