Analysis of mouse intestinal organoid culture with conditioned media isolated from mucosal enteric glial cells

This protocol describes the isolation and culture of 3D intestinal crypt organoids with stromal niche cells. We show a murine organoid culture system that utilizes conditioned media isolated from primary, mucosal enteric glial cell culture. We describe three assays of analyzing this organoid culture...

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Detalles Bibliográficos
Autores principales: Baghdadi, Meryem B., Kim, Tae-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062428/
https://www.ncbi.nlm.nih.gov/pubmed/35516847
http://dx.doi.org/10.1016/j.xpro.2022.101351

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9062428/
https://www.ncbi.nlm.nih.gov/pubmed/35516847
http://dx.doi.org/10.1016/j.xpro.2022.101351

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