Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

Herein, silica nanoparticles were synthesized and chemically modified with iminodiacetic acid (IDA) and Ni(2+) ions surrounded by a bis-acrylamide polymeric shell to obtain a new core–shell immobilized metal affinity chromatography (IMAC) based material. These Ni(2+)–IDA-core–shell silica nanopartic...

Descripción completa

Detalles Bibliográficos
Autores principales: Hernandez-Leon, Sergio G., Sarabia-Sainz, Jose A., Ramos-Clamont Montfort, Gabriela, Huerta-Ocampo, José Ángel, Guzman-Partida, Ana M., Robles-Burgueño, Maria del Refugio, Burgara-Estrella, Alexel J., Vazquez-Moreno, Luz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063014/
https://www.ncbi.nlm.nih.gov/pubmed/35520222
http://dx.doi.org/10.1039/c9ra01144g

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063014/
https://www.ncbi.nlm.nih.gov/pubmed/35520222
http://dx.doi.org/10.1039/c9ra01144g

Ejemplares similares