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Bifunctional nickel–iminodiacetic acid-core–shell silica nanoparticles for the exclusion of high molecular weight proteins and purification of His-tagged recombinant proteins

Herein, silica nanoparticles were synthesized and chemically modified with iminodiacetic acid (IDA) and Ni(2+) ions surrounded by a bis-acrylamide polymeric shell to obtain a new core–shell immobilized metal affinity chromatography (IMAC) based material. These Ni(2+)–IDA-core–shell silica nanopartic...

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Detalles Bibliográficos
Autores principales:	Hernandez-Leon, Sergio G., Sarabia-Sainz, Jose A., Ramos-Clamont Montfort, Gabriela, Huerta-Ocampo, José Ángel, Guzman-Partida, Ana M., Robles-Burgueño, Maria del Refugio, Burgara-Estrella, Alexel J., Vazquez-Moreno, Luz
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Royal Society of Chemistry 2019
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063014/ https://www.ncbi.nlm.nih.gov/pubmed/35520222 http://dx.doi.org/10.1039/c9ra01144g

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