Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites

BACKGROUND: 16S rDNA-PCR for the identification of a bacterial species is an established method. However, the DNA extraction reagents as well as the PCR reagents may contain residual bacterial DNA, which consequently generates false-positive PCR results. Additionally, previously used methods are fre...

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Detalles Bibliográficos
Autores principales: Egli, Konrad, Risch, Martin, Risch, Lorenz, Bodmer, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063205/
https://www.ncbi.nlm.nih.gov/pubmed/35501697
http://dx.doi.org/10.1186/s12866-022-02542-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063205/
https://www.ncbi.nlm.nih.gov/pubmed/35501697
http://dx.doi.org/10.1186/s12866-022-02542-w

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