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Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles
Despite a few decades of research, interest continues in understanding the potential influences of low energy microwave irradiation on biological systems. In the present study, growth of E. coli DE3 in LB media slowed in the presence of microwave irradiation (max. 10 W) while the temperature of cult...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063421/ https://www.ncbi.nlm.nih.gov/pubmed/35517035 http://dx.doi.org/10.1039/c9ra00617f |
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author | Mazinani, Sina Atrin Noaman, Nour Pergande, Melissa R. Cologna, Stephanie M. Coorssen, Jens Yan, Hongbin |
author_facet | Mazinani, Sina Atrin Noaman, Nour Pergande, Melissa R. Cologna, Stephanie M. Coorssen, Jens Yan, Hongbin |
author_sort | Mazinani, Sina Atrin |
collection | PubMed |
description | Despite a few decades of research, interest continues in understanding the potential influences of low energy microwave irradiation on biological systems. In the present study, growth of E. coli DE3 in LB media slowed in the presence of microwave irradiation (max. 10 W) while the temperature of cultures was maintained at 37 °C. Viable cell counts in microwave-irradiated cultures were also significantly lower. When microwave irradiation was ceased, E. coli growth was restored. A top-down proteomic analysis of total proteins isolated from control and microwave-irradiated E. coli cultures revealed differential abundance of 10 resolved protein spots, with multiple proteins identified in each following mass spectrometric analysis. Among these proteins, a number are involved in metabolism, suggesting alterations to metabolic activities following microwave irradiation. Furthermore, four amino acid-tRNA ligases were also identified, pointing to the possibility of stress responses in E. coli under microwave irradiation. |
format | Online Article Text |
id | pubmed-9063421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90634212022-05-04 Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles Mazinani, Sina Atrin Noaman, Nour Pergande, Melissa R. Cologna, Stephanie M. Coorssen, Jens Yan, Hongbin RSC Adv Chemistry Despite a few decades of research, interest continues in understanding the potential influences of low energy microwave irradiation on biological systems. In the present study, growth of E. coli DE3 in LB media slowed in the presence of microwave irradiation (max. 10 W) while the temperature of cultures was maintained at 37 °C. Viable cell counts in microwave-irradiated cultures were also significantly lower. When microwave irradiation was ceased, E. coli growth was restored. A top-down proteomic analysis of total proteins isolated from control and microwave-irradiated E. coli cultures revealed differential abundance of 10 resolved protein spots, with multiple proteins identified in each following mass spectrometric analysis. Among these proteins, a number are involved in metabolism, suggesting alterations to metabolic activities following microwave irradiation. Furthermore, four amino acid-tRNA ligases were also identified, pointing to the possibility of stress responses in E. coli under microwave irradiation. The Royal Society of Chemistry 2019-04-16 /pmc/articles/PMC9063421/ /pubmed/35517035 http://dx.doi.org/10.1039/c9ra00617f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Mazinani, Sina Atrin Noaman, Nour Pergande, Melissa R. Cologna, Stephanie M. Coorssen, Jens Yan, Hongbin Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles |
title | Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles |
title_full | Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles |
title_fullStr | Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles |
title_full_unstemmed | Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles |
title_short | Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles |
title_sort | exposure to microwave irradiation at constant culture temperature slows the growth of escherichia coli de3 cells, leading to modified proteomic profiles |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063421/ https://www.ncbi.nlm.nih.gov/pubmed/35517035 http://dx.doi.org/10.1039/c9ra00617f |
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