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Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles

Despite a few decades of research, interest continues in understanding the potential influences of low energy microwave irradiation on biological systems. In the present study, growth of E. coli DE3 in LB media slowed in the presence of microwave irradiation (max. 10 W) while the temperature of cult...

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Autores principales: Mazinani, Sina Atrin, Noaman, Nour, Pergande, Melissa R., Cologna, Stephanie M., Coorssen, Jens, Yan, Hongbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063421/
https://www.ncbi.nlm.nih.gov/pubmed/35517035
http://dx.doi.org/10.1039/c9ra00617f
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author Mazinani, Sina Atrin
Noaman, Nour
Pergande, Melissa R.
Cologna, Stephanie M.
Coorssen, Jens
Yan, Hongbin
author_facet Mazinani, Sina Atrin
Noaman, Nour
Pergande, Melissa R.
Cologna, Stephanie M.
Coorssen, Jens
Yan, Hongbin
author_sort Mazinani, Sina Atrin
collection PubMed
description Despite a few decades of research, interest continues in understanding the potential influences of low energy microwave irradiation on biological systems. In the present study, growth of E. coli DE3 in LB media slowed in the presence of microwave irradiation (max. 10 W) while the temperature of cultures was maintained at 37 °C. Viable cell counts in microwave-irradiated cultures were also significantly lower. When microwave irradiation was ceased, E. coli growth was restored. A top-down proteomic analysis of total proteins isolated from control and microwave-irradiated E. coli cultures revealed differential abundance of 10 resolved protein spots, with multiple proteins identified in each following mass spectrometric analysis. Among these proteins, a number are involved in metabolism, suggesting alterations to metabolic activities following microwave irradiation. Furthermore, four amino acid-tRNA ligases were also identified, pointing to the possibility of stress responses in E. coli under microwave irradiation.
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spelling pubmed-90634212022-05-04 Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles Mazinani, Sina Atrin Noaman, Nour Pergande, Melissa R. Cologna, Stephanie M. Coorssen, Jens Yan, Hongbin RSC Adv Chemistry Despite a few decades of research, interest continues in understanding the potential influences of low energy microwave irradiation on biological systems. In the present study, growth of E. coli DE3 in LB media slowed in the presence of microwave irradiation (max. 10 W) while the temperature of cultures was maintained at 37 °C. Viable cell counts in microwave-irradiated cultures were also significantly lower. When microwave irradiation was ceased, E. coli growth was restored. A top-down proteomic analysis of total proteins isolated from control and microwave-irradiated E. coli cultures revealed differential abundance of 10 resolved protein spots, with multiple proteins identified in each following mass spectrometric analysis. Among these proteins, a number are involved in metabolism, suggesting alterations to metabolic activities following microwave irradiation. Furthermore, four amino acid-tRNA ligases were also identified, pointing to the possibility of stress responses in E. coli under microwave irradiation. The Royal Society of Chemistry 2019-04-16 /pmc/articles/PMC9063421/ /pubmed/35517035 http://dx.doi.org/10.1039/c9ra00617f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Mazinani, Sina Atrin
Noaman, Nour
Pergande, Melissa R.
Cologna, Stephanie M.
Coorssen, Jens
Yan, Hongbin
Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles
title Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles
title_full Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles
title_fullStr Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles
title_full_unstemmed Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles
title_short Exposure to microwave irradiation at constant culture temperature slows the growth of Escherichia coli DE3 cells, leading to modified proteomic profiles
title_sort exposure to microwave irradiation at constant culture temperature slows the growth of escherichia coli de3 cells, leading to modified proteomic profiles
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9063421/
https://www.ncbi.nlm.nih.gov/pubmed/35517035
http://dx.doi.org/10.1039/c9ra00617f
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