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Mapping transgene insertion sites reveals the α-Cre transgene expression in both developing retina and olfactory neurons
The Tg(Pax6-cre,GFP)(2Pgr) (α-Cre) mouse is a commonly used Cre line thought to be retinal-specific. Using targeted locus amplification (TLA), we mapped the insertion site of the transgene, and defined primers useful to deduce zygosity. Further analyses revealed four tandem copies of the transgene....
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065156/ https://www.ncbi.nlm.nih.gov/pubmed/35505181 http://dx.doi.org/10.1038/s42003-022-03379-9 |