Isolation of human intrahepatic leukocytes for phenotypic and functional characterization by flow cytometry

With the growing appreciation of tissue-resident immunity, studying tissue-specific immune cells contributing to both homeostasis and disease is imperative. Here, we provide a protocol for the isolation of human intrahepatic leukocytes (IHL) maximizing viability, purity, and yield. Our protocol is s...

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Detalles Bibliográficos
Autores principales: Kucykowicz, Stephanie, Amin, Oliver E., Burton, Alice R., Swadling, Leo, Schmidt, Nathalie M., Zakeri, Nekisa, Davies, Jessica, Aidoo-Micah, Gloryanne, Stegmann, Kerstin A., Easom, Nicholas J., Jeffery-Smith, Anna, Maini, Mala K., Pallett, Laura J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065431/
https://www.ncbi.nlm.nih.gov/pubmed/35516846
http://dx.doi.org/10.1016/j.xpro.2022.101356

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065431/
https://www.ncbi.nlm.nih.gov/pubmed/35516846
http://dx.doi.org/10.1016/j.xpro.2022.101356

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