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Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study
This study determines the effect of cookies made from sea grapes (Caulerpa racemosa) on PGC-1α, total cholesterol, and blood glucose levels on mice fed with a Cholesterol- and Fat-Enriched Diet (CFED). The antioxidant activity, tyrosinase inhibition, α-glucosidase, and α-amylase inhibition is also a...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065618/ https://www.ncbi.nlm.nih.gov/pubmed/35521505 http://dx.doi.org/10.1016/j.heliyon.2022.e09348 |
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author | Ngadiarti, Iskari Nurkolis, Fahrul Handoko, Matthew Nathaniel Perdana, Fachruddin Permatasari, Happy Kurnia Taslim, Nurpudji Astuti Mayulu, Nelly Wewengkang, Defny Silvia Noor, Sutamara Lasurdi Batubara, Siti Chairiyah Tanner, Melvin Junior Sabrina, Nindy |
author_facet | Ngadiarti, Iskari Nurkolis, Fahrul Handoko, Matthew Nathaniel Perdana, Fachruddin Permatasari, Happy Kurnia Taslim, Nurpudji Astuti Mayulu, Nelly Wewengkang, Defny Silvia Noor, Sutamara Lasurdi Batubara, Siti Chairiyah Tanner, Melvin Junior Sabrina, Nindy |
author_sort | Ngadiarti, Iskari |
collection | PubMed |
description | This study determines the effect of cookies made from sea grapes (Caulerpa racemosa) on PGC-1α, total cholesterol, and blood glucose levels on mice fed with a Cholesterol- and Fat-Enriched Diet (CFED). The antioxidant activity, tyrosinase inhibition, α-glucosidase, and α-amylase inhibition is also analyzed in order to assess the in vitro anti-aging potential of sea grapes cookies. Forty male Mus muscullus albino mice weighing 20 g–30 g were used and randomly distributed into four groups of ten animals each. Group A served as a normal control (given a standard dry pellet diet), Group B was given CFED only, and mice in Groups C and D were given CFED with 100 mg and 200 mg/20 g body weight of sea grapes cookies, respectively for 4 weeks. In vitro study shows that the percentage of inhibition activity of antioxidant, L-Tyrosine, L-Dopa, α-glucosidase, and α-amylase inhibition were 45.65 ± 1.50, 8.95 ± 0.06, 21.31 ± 0.98, 77.12 ± 4.67 and 70.94 ± 0.98, respectively. This study found that group D had better activity in lowering blood glucose than group C (p < 0.0001). In addition, although there was not found significant difference between groups C and D in blood cholesterol reduction and PGC-1α (p = 0.1482), both groups experienced the same effect in total cholesterol reduction and PGC-1α in mice (significantly, p < 0001). Thus, we conclude that sea grapes cookies are proven to improve PGC-1α, total cholesterol, and blood glucose levels in mice fed with CFED. Hence, sea grapes cookies is a potential anti-aging novel-functional food. |
format | Online Article Text |
id | pubmed-9065618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90656182022-05-04 Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study Ngadiarti, Iskari Nurkolis, Fahrul Handoko, Matthew Nathaniel Perdana, Fachruddin Permatasari, Happy Kurnia Taslim, Nurpudji Astuti Mayulu, Nelly Wewengkang, Defny Silvia Noor, Sutamara Lasurdi Batubara, Siti Chairiyah Tanner, Melvin Junior Sabrina, Nindy Heliyon Research Article This study determines the effect of cookies made from sea grapes (Caulerpa racemosa) on PGC-1α, total cholesterol, and blood glucose levels on mice fed with a Cholesterol- and Fat-Enriched Diet (CFED). The antioxidant activity, tyrosinase inhibition, α-glucosidase, and α-amylase inhibition is also analyzed in order to assess the in vitro anti-aging potential of sea grapes cookies. Forty male Mus muscullus albino mice weighing 20 g–30 g were used and randomly distributed into four groups of ten animals each. Group A served as a normal control (given a standard dry pellet diet), Group B was given CFED only, and mice in Groups C and D were given CFED with 100 mg and 200 mg/20 g body weight of sea grapes cookies, respectively for 4 weeks. In vitro study shows that the percentage of inhibition activity of antioxidant, L-Tyrosine, L-Dopa, α-glucosidase, and α-amylase inhibition were 45.65 ± 1.50, 8.95 ± 0.06, 21.31 ± 0.98, 77.12 ± 4.67 and 70.94 ± 0.98, respectively. This study found that group D had better activity in lowering blood glucose than group C (p < 0.0001). In addition, although there was not found significant difference between groups C and D in blood cholesterol reduction and PGC-1α (p = 0.1482), both groups experienced the same effect in total cholesterol reduction and PGC-1α in mice (significantly, p < 0001). Thus, we conclude that sea grapes cookies are proven to improve PGC-1α, total cholesterol, and blood glucose levels in mice fed with CFED. Hence, sea grapes cookies is a potential anti-aging novel-functional food. Elsevier 2022-04-28 /pmc/articles/PMC9065618/ /pubmed/35521505 http://dx.doi.org/10.1016/j.heliyon.2022.e09348 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Ngadiarti, Iskari Nurkolis, Fahrul Handoko, Matthew Nathaniel Perdana, Fachruddin Permatasari, Happy Kurnia Taslim, Nurpudji Astuti Mayulu, Nelly Wewengkang, Defny Silvia Noor, Sutamara Lasurdi Batubara, Siti Chairiyah Tanner, Melvin Junior Sabrina, Nindy Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study |
title | Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study |
title_full | Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study |
title_fullStr | Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study |
title_full_unstemmed | Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study |
title_short | Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study |
title_sort | anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065618/ https://www.ncbi.nlm.nih.gov/pubmed/35521505 http://dx.doi.org/10.1016/j.heliyon.2022.e09348 |
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