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A novel methodological approach to simultaneously extract high-quality total RNA and proteins from cortical and trabecular bone

Molecular differences between cortical and trabecular bone, of relevance to understanding the pathophysiological basis of bone diseases, can be determined only through effective isolation methods for RNA and proteins. Here we present a TRIzol-based method, which combines bone pulverization and homog...

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Detalles Bibliográficos
Autores principales: Faraldi, Martina, Mangiavini, Laura, Conte, Caterina, Banfi, Giuseppe, Napoli, Nicola, Lombardi, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065961/
https://www.ncbi.nlm.nih.gov/pubmed/35506206
http://dx.doi.org/10.1098/rsob.210387