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The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis
The transcription factor complex, consisting of LMO2, TAL1 or LYL1, and GATA2, plays an important role in capillary sprouting by regulating VEGFR2, DLL4, and angiopoietin 2 in tip cells. Overexpression of the basic helix-loop-helix transcription factor LYL1 in transgenic mice results in shortened ta...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068620/ https://www.ncbi.nlm.nih.gov/pubmed/35508511 http://dx.doi.org/10.1038/s41598-022-11297-3 |
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author | Yamada, Yoshihiro Zhong, Yi Miki, Shiho Taura, Akiko Rabbitts, Terence H. |
author_facet | Yamada, Yoshihiro Zhong, Yi Miki, Shiho Taura, Akiko Rabbitts, Terence H. |
author_sort | Yamada, Yoshihiro |
collection | PubMed |
description | The transcription factor complex, consisting of LMO2, TAL1 or LYL1, and GATA2, plays an important role in capillary sprouting by regulating VEGFR2, DLL4, and angiopoietin 2 in tip cells. Overexpression of the basic helix-loop-helix transcription factor LYL1 in transgenic mice results in shortened tails. This phenotype is associated with vessel hyperbranching and a relative paucity of straight vessels due to DLL4 downregulation in tip cells by forming aberrant complex consisting of LMO2 and LYL1. Knockdown of LMO2 or TAL1 inhibits capillary sprouting in spheroid-based angiogenesis assays, which is associated with decreased angiopoietin 2 secretion. In the same assay using mixed TAL1- and LYL1-expressing endothelial cells, TAL1 was found to be primarily located in tip cells, while LYL1-expressing cells tended to occupy the stalk position in sprouts by upregulating VEGFR1 than TAL1. Thus, the interaction between LMO2 and TAL1 in tip cells plays a key role in angiogenic switch of sprouting angiogenesis. |
format | Online Article Text |
id | pubmed-9068620 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90686202022-05-05 The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis Yamada, Yoshihiro Zhong, Yi Miki, Shiho Taura, Akiko Rabbitts, Terence H. Sci Rep Article The transcription factor complex, consisting of LMO2, TAL1 or LYL1, and GATA2, plays an important role in capillary sprouting by regulating VEGFR2, DLL4, and angiopoietin 2 in tip cells. Overexpression of the basic helix-loop-helix transcription factor LYL1 in transgenic mice results in shortened tails. This phenotype is associated with vessel hyperbranching and a relative paucity of straight vessels due to DLL4 downregulation in tip cells by forming aberrant complex consisting of LMO2 and LYL1. Knockdown of LMO2 or TAL1 inhibits capillary sprouting in spheroid-based angiogenesis assays, which is associated with decreased angiopoietin 2 secretion. In the same assay using mixed TAL1- and LYL1-expressing endothelial cells, TAL1 was found to be primarily located in tip cells, while LYL1-expressing cells tended to occupy the stalk position in sprouts by upregulating VEGFR1 than TAL1. Thus, the interaction between LMO2 and TAL1 in tip cells plays a key role in angiogenic switch of sprouting angiogenesis. Nature Publishing Group UK 2022-05-04 /pmc/articles/PMC9068620/ /pubmed/35508511 http://dx.doi.org/10.1038/s41598-022-11297-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yamada, Yoshihiro Zhong, Yi Miki, Shiho Taura, Akiko Rabbitts, Terence H. The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis |
title | The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis |
title_full | The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis |
title_fullStr | The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis |
title_full_unstemmed | The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis |
title_short | The transcription factor complex LMO2/TAL1 regulates branching and endothelial cell migration in sprouting angiogenesis |
title_sort | transcription factor complex lmo2/tal1 regulates branching and endothelial cell migration in sprouting angiogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068620/ https://www.ncbi.nlm.nih.gov/pubmed/35508511 http://dx.doi.org/10.1038/s41598-022-11297-3 |
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