Cargando…

A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells

Human NAD(P)H:quinone oxidoreductase (hNQO1) can be used as a biomarker for the early diagnosis of cancer. In this article, a novel fluorescent probe 1 for detecting hNQO1 was designed and synthesized by using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) derivative (TCF-OH) as a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Ya, Han, Jialing, Zhang, Qian, Zhao, Zhou, Wang, Jin, Xu, Xiaowei, Hao, Haiping, Zhang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070553/
https://www.ncbi.nlm.nih.gov/pubmed/35528556
http://dx.doi.org/10.1039/c9ra05650e
_version_ 1784700665295011840
author Zhu, Ya
Han, Jialing
Zhang, Qian
Zhao, Zhou
Wang, Jin
Xu, Xiaowei
Hao, Haiping
Zhang, Jun
author_facet Zhu, Ya
Han, Jialing
Zhang, Qian
Zhao, Zhou
Wang, Jin
Xu, Xiaowei
Hao, Haiping
Zhang, Jun
author_sort Zhu, Ya
collection PubMed
description Human NAD(P)H:quinone oxidoreductase (hNQO1) can be used as a biomarker for the early diagnosis of cancer. In this article, a novel fluorescent probe 1 for detecting hNQO1 was designed and synthesized by using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) derivative (TCF-OH) as a fluorophore and quinone propionic acid (QPA) as a recognition group. The probe, which has high selectivity for hNQO1 and a Stokes shift of about 117 nm, shows a linear relationship with hNQO1 concentrations in the range of 0.25–3 μg ml(−1) with the color changes from yellow to red and was successfully applied to intracellular hNQO1 imaging.
format Online
Article
Text
id pubmed-9070553
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90705532022-05-05 A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells Zhu, Ya Han, Jialing Zhang, Qian Zhao, Zhou Wang, Jin Xu, Xiaowei Hao, Haiping Zhang, Jun RSC Adv Chemistry Human NAD(P)H:quinone oxidoreductase (hNQO1) can be used as a biomarker for the early diagnosis of cancer. In this article, a novel fluorescent probe 1 for detecting hNQO1 was designed and synthesized by using 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) derivative (TCF-OH) as a fluorophore and quinone propionic acid (QPA) as a recognition group. The probe, which has high selectivity for hNQO1 and a Stokes shift of about 117 nm, shows a linear relationship with hNQO1 concentrations in the range of 0.25–3 μg ml(−1) with the color changes from yellow to red and was successfully applied to intracellular hNQO1 imaging. The Royal Society of Chemistry 2019-08-27 /pmc/articles/PMC9070553/ /pubmed/35528556 http://dx.doi.org/10.1039/c9ra05650e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhu, Ya
Han, Jialing
Zhang, Qian
Zhao, Zhou
Wang, Jin
Xu, Xiaowei
Hao, Haiping
Zhang, Jun
A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells
title A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells
title_full A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells
title_fullStr A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells
title_full_unstemmed A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells
title_short A highly selective fluorescent probe for human NAD(P)H:quinone oxidoreductase 1 (hNQO1) detection and imaging in living tumor cells
title_sort highly selective fluorescent probe for human nad(p)h:quinone oxidoreductase 1 (hnqo1) detection and imaging in living tumor cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070553/
https://www.ncbi.nlm.nih.gov/pubmed/35528556
http://dx.doi.org/10.1039/c9ra05650e
work_keys_str_mv AT zhuya ahighlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT hanjialing ahighlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT zhangqian ahighlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT zhaozhou ahighlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT wangjin ahighlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT xuxiaowei ahighlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT haohaiping ahighlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT zhangjun ahighlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT zhuya highlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT hanjialing highlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT zhangqian highlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT zhaozhou highlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT wangjin highlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT xuxiaowei highlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT haohaiping highlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells
AT zhangjun highlyselectivefluorescentprobeforhumannadphquinoneoxidoreductase1hnqo1detectionandimaginginlivingtumorcells