Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system

We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3-ITD protein was expressed in Bombyx mori L. pupae in...

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Detalles Bibliográficos
Autores principales: Yamamoto, Naoki, Kikuchi, Jiro, Furukawa, Yusuke, Shibayama, Naoya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070948/
https://www.ncbi.nlm.nih.gov/pubmed/35511790
http://dx.doi.org/10.1371/journal.pone.0261699

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070948/
https://www.ncbi.nlm.nih.gov/pubmed/35511790
http://dx.doi.org/10.1371/journal.pone.0261699

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