Rational guide RNA engineering for small-molecule control of CRISPR/Cas9 and gene editing

It is important to control CRISPR/Cas9 when sufficient editing is obtained. In the current study, rational engineering of guide RNAs (gRNAs) is performed to develop small-molecule-responsive CRISPR/Cas9. For our purpose, the sequence of gRNAs are modified to introduce ligand binding sites based on t...

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Detalles Bibliográficos
Autores principales: Liu, Xingyu, Xiong, Wei, Qi, Qianqian, Zhang, Yutong, Ji, Huimin, Cui, Shuangyu, An, Jing, Sun, Xiaoming, Yin, Hao, Tian, Tian, Zhou, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071477/
https://www.ncbi.nlm.nih.gov/pubmed/35446403
http://dx.doi.org/10.1093/nar/gkac255

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071477/
https://www.ncbi.nlm.nih.gov/pubmed/35446403
http://dx.doi.org/10.1093/nar/gkac255

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