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PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements

Ultra-multiplexed fluorescence imaging requires the use of spectrally overlapping fluorophores to label proteins and then to unmix the images of the fluorophores. However, doing this remains a challenge, especially in highly heterogeneous specimens, such as the brain, owing to the high degree of var...

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Detalles Bibliográficos
Autores principales: Seo, Junyoung, Sim, Yeonbo, Kim, Jeewon, Kim, Hyunwoo, Cho, In, Nam, Hoyeon, Yoon, Young-Gyu, Chang, Jae-Byum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072354/
https://www.ncbi.nlm.nih.gov/pubmed/35513404
http://dx.doi.org/10.1038/s41467-022-30168-z