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The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate

Oysters, favored as a delicious seafood by people worldwide, contain various nutritional components, especially proteins. In this work, the immunoregulatory peptides were isolated and purified from oyster enzymatic hydrolysate by utilizing anion exchange chromatography and RP-HPLC, and were identifi...

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Detalles Bibliográficos
Autores principales: Li, Wan, Xu, Cheng, Zhang, Chaohua, Cao, Wenhong, Qin, Xiaoming, Gao, Jialong, Zheng, Huina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073163/
https://www.ncbi.nlm.nih.gov/pubmed/35529727
http://dx.doi.org/10.1039/c9ra04255e
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author Li, Wan
Xu, Cheng
Zhang, Chaohua
Cao, Wenhong
Qin, Xiaoming
Gao, Jialong
Zheng, Huina
author_facet Li, Wan
Xu, Cheng
Zhang, Chaohua
Cao, Wenhong
Qin, Xiaoming
Gao, Jialong
Zheng, Huina
author_sort Li, Wan
collection PubMed
description Oysters, favored as a delicious seafood by people worldwide, contain various nutritional components, especially proteins. In this work, the immunoregulatory peptides were isolated and purified from oyster enzymatic hydrolysate by utilizing anion exchange chromatography and RP-HPLC, and were identified via LC-MS/MS. A proliferation assay, a phagocytosis assay, and TNF-α production, NO production and IL-6 production assays were used to examine the immunoregulatory effects of the two identified peptides. The results indicate that the peptides, DNSIAMESMK (P1) and LLQLGSGR (P2), increased the proliferation rate of macrophages, TNF-α and NO production, IL-6 production, and the phagocytosis ability, but to different degrees; P2, with more hydrophobic amino acids, was more potent than P1. This suggested that these peptides might be potential candidates for developing immunoregulatory functional foods and nutraceuticals.
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spelling pubmed-90731632022-05-06 The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate Li, Wan Xu, Cheng Zhang, Chaohua Cao, Wenhong Qin, Xiaoming Gao, Jialong Zheng, Huina RSC Adv Chemistry Oysters, favored as a delicious seafood by people worldwide, contain various nutritional components, especially proteins. In this work, the immunoregulatory peptides were isolated and purified from oyster enzymatic hydrolysate by utilizing anion exchange chromatography and RP-HPLC, and were identified via LC-MS/MS. A proliferation assay, a phagocytosis assay, and TNF-α production, NO production and IL-6 production assays were used to examine the immunoregulatory effects of the two identified peptides. The results indicate that the peptides, DNSIAMESMK (P1) and LLQLGSGR (P2), increased the proliferation rate of macrophages, TNF-α and NO production, IL-6 production, and the phagocytosis ability, but to different degrees; P2, with more hydrophobic amino acids, was more potent than P1. This suggested that these peptides might be potential candidates for developing immunoregulatory functional foods and nutraceuticals. The Royal Society of Chemistry 2019-10-15 /pmc/articles/PMC9073163/ /pubmed/35529727 http://dx.doi.org/10.1039/c9ra04255e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Li, Wan
Xu, Cheng
Zhang, Chaohua
Cao, Wenhong
Qin, Xiaoming
Gao, Jialong
Zheng, Huina
The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate
title The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate
title_full The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate
title_fullStr The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate
title_full_unstemmed The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate
title_short The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate
title_sort purification and identification of immunoregulatory peptides from oyster (crassostrea hongkongensis) enzymatic hydrolysate
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073163/
https://www.ncbi.nlm.nih.gov/pubmed/35529727
http://dx.doi.org/10.1039/c9ra04255e
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