Cargando…
Enhanced extracellular recombinant keratinase activity in Bacillus subtilis SCK6 through signal peptide optimization and site-directed mutagenesis
Keratinase has a great commercial value owing to its applications in the enzymatic dehairing of goatskins. In this study, we adopted a combined strategy to enhance the extracellular recombinant keratinase activity in Bacillus subtilis SCK6. First, nine signal peptides were screened to enhance the ex...
Autores principales: | Tian, Jiewei, Long, Xiufeng, Tian, Yongqiang, Shi, Bi |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073338/ https://www.ncbi.nlm.nih.gov/pubmed/35529123 http://dx.doi.org/10.1039/c9ra07866e |
Ejemplares similares
-
Heterologous Expression of Recombinant Transglutaminase in Bacillus subtilis SCK6 with Optimized Signal Peptide and Codon, and Its Impact on Gelatin Properties
por: Wang, Shiting, et al.
Publicado: (2020) -
Enhanced and Secretory Expression of Human Granulocyte Colony Stimulating Factor by Bacillus subtilis SCK6
por: Bashir, Shaista, et al.
Publicado: (2015) -
Structural characterization and active site prediction of keratinase from Bacillus flexus
por: Padmavathi, Arumugam, et al.
Publicado: (2022) -
Recombinant expression and biochemical characterization of a novel keratinase BsKER71 from feather degrading bacterium Bacillus subtilis S1-4
por: Yong, Bin, et al.
Publicado: (2020) -
Enhancement of feather degrading keratinase of Streptomyces swerraensis KN23, applying mutagenesis and statistical optimization to improve keratinase activity
por: Abd El-Aziz, Nagwa M., et al.
Publicado: (2023)