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Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA)
MAIN CONCLUSION: α-Amylase synthesis by wheat aleurone during grain development (late maturity α-amylase) appears to be independent of gibberellin unlike α-amylase synthesis by aleurone during germination or following treatment with exogenous GA. ABSTRACT: Late-maturity α-amylase (LMA) in wheat (Tri...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076747/ https://www.ncbi.nlm.nih.gov/pubmed/35522329 http://dx.doi.org/10.1007/s00425-022-03899-y |
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author | Mares, Daryl Derkx, Adinda Cheong, Judy Zaharia, Irina Asenstorfer, Robert Mrva, Kolumbina |
author_facet | Mares, Daryl Derkx, Adinda Cheong, Judy Zaharia, Irina Asenstorfer, Robert Mrva, Kolumbina |
author_sort | Mares, Daryl |
collection | PubMed |
description | MAIN CONCLUSION: α-Amylase synthesis by wheat aleurone during grain development (late maturity α-amylase) appears to be independent of gibberellin unlike α-amylase synthesis by aleurone during germination or following treatment with exogenous GA. ABSTRACT: Late-maturity α-amylase (LMA) in wheat (Triticum aestivum L.) involves the synthesis of α-amylase by the aleurone tissue during grain development. Previous research identified a putative ent-copalyl diphosphate synthase gene, coding for an enzyme that controls the first step in gibberellin biosynthesis, that underlies the major genetic locus involved in variation in LMA phenotype. The reported results for gene transcript analysis, preliminary gibberellin analysis and the effects of DELLA mutants on LMA phenotype appeared to be consistent with involvement of gibberellin but did not provide definitive proof of a causal link. Conversely, several observations do not appear to be consistent with this hypothesis. In this current study, LMA phenotype, gibberellin profiles and ABA content were recorded for experiments involving susceptible and resistant genotypes, gibberellin biosynthesis inhibitors, genetic lines containing different LMA quantitative trait loci and treatment of distal halves of developing grains with exogenous gibberellin. The results suggested that gibberellin may not be a prerequisite for LMA expression and further that the mechanism involved in triggering α-amylase synthesis did not correspond to the model proposed for germination and gibberellin challenged aleurone of ripe grain. The results provide new insight into LMA and highlight the need to investigate alternate pathways for the induction of α-amylase gene transcription, the function of novel 1-β-OH gibberellins and other functions of DELLA proteins in developing grains. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00425-022-03899-y. |
format | Online Article Text |
id | pubmed-9076747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-90767472022-05-08 Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA) Mares, Daryl Derkx, Adinda Cheong, Judy Zaharia, Irina Asenstorfer, Robert Mrva, Kolumbina Planta Original Article MAIN CONCLUSION: α-Amylase synthesis by wheat aleurone during grain development (late maturity α-amylase) appears to be independent of gibberellin unlike α-amylase synthesis by aleurone during germination or following treatment with exogenous GA. ABSTRACT: Late-maturity α-amylase (LMA) in wheat (Triticum aestivum L.) involves the synthesis of α-amylase by the aleurone tissue during grain development. Previous research identified a putative ent-copalyl diphosphate synthase gene, coding for an enzyme that controls the first step in gibberellin biosynthesis, that underlies the major genetic locus involved in variation in LMA phenotype. The reported results for gene transcript analysis, preliminary gibberellin analysis and the effects of DELLA mutants on LMA phenotype appeared to be consistent with involvement of gibberellin but did not provide definitive proof of a causal link. Conversely, several observations do not appear to be consistent with this hypothesis. In this current study, LMA phenotype, gibberellin profiles and ABA content were recorded for experiments involving susceptible and resistant genotypes, gibberellin biosynthesis inhibitors, genetic lines containing different LMA quantitative trait loci and treatment of distal halves of developing grains with exogenous gibberellin. The results suggested that gibberellin may not be a prerequisite for LMA expression and further that the mechanism involved in triggering α-amylase synthesis did not correspond to the model proposed for germination and gibberellin challenged aleurone of ripe grain. The results provide new insight into LMA and highlight the need to investigate alternate pathways for the induction of α-amylase gene transcription, the function of novel 1-β-OH gibberellins and other functions of DELLA proteins in developing grains. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00425-022-03899-y. Springer Berlin Heidelberg 2022-05-06 2022 /pmc/articles/PMC9076747/ /pubmed/35522329 http://dx.doi.org/10.1007/s00425-022-03899-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Mares, Daryl Derkx, Adinda Cheong, Judy Zaharia, Irina Asenstorfer, Robert Mrva, Kolumbina Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA) |
title | Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA) |
title_full | Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA) |
title_fullStr | Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA) |
title_full_unstemmed | Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA) |
title_short | Gibberellins in developing wheat grains and their relationship to late maturity α-amylase (LMA) |
title_sort | gibberellins in developing wheat grains and their relationship to late maturity α-amylase (lma) |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076747/ https://www.ncbi.nlm.nih.gov/pubmed/35522329 http://dx.doi.org/10.1007/s00425-022-03899-y |
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