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Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells

The Hippo pathway plays critical roles in cell growth, differentiation, development, tissue homeostasis, and cancer. Here, we describe protocols to measure phosphorylation, subcellular localization, and kinase activity for core Hippo pathway components YAP and LATS. The phos-tag technology is partic...

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Detalles Bibliográficos
Autores principales: Luo, Min, Hu, Yuan, Wei, Xiawei, Guan, Kun-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076959/
https://www.ncbi.nlm.nih.gov/pubmed/35535164
http://dx.doi.org/10.1016/j.xpro.2021.101102