Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells

The Hippo pathway plays critical roles in cell growth, differentiation, development, tissue homeostasis, and cancer. Here, we describe protocols to measure phosphorylation, subcellular localization, and kinase activity for core Hippo pathway components YAP and LATS. The phos-tag technology is partic...

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Autores principales: Luo, Min, Hu, Yuan, Wei, Xiawei, Guan, Kun-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076959/
https://www.ncbi.nlm.nih.gov/pubmed/35535164
http://dx.doi.org/10.1016/j.xpro.2021.101102
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author Luo, Min
Hu, Yuan
Wei, Xiawei
Guan, Kun-Liang
author_facet Luo, Min
Hu, Yuan
Wei, Xiawei
Guan, Kun-Liang
author_sort Luo, Min
collection PubMed
description The Hippo pathway plays critical roles in cell growth, differentiation, development, tissue homeostasis, and cancer. Here, we describe protocols to measure phosphorylation, subcellular localization, and kinase activity for core Hippo pathway components YAP and LATS. The phos-tag technology is particularly useful to quantitatively detect protein phosphorylation even without a phosphospecific antibody. We use HEK293A cells as an example, but the protocols can be applied to other cell lines and tissues. For complete details on the use and execution of this profile, please refer to Luo et al. (2020).
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spelling pubmed-90769592022-05-08 Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells Luo, Min Hu, Yuan Wei, Xiawei Guan, Kun-Liang STAR Protoc Protocol The Hippo pathway plays critical roles in cell growth, differentiation, development, tissue homeostasis, and cancer. Here, we describe protocols to measure phosphorylation, subcellular localization, and kinase activity for core Hippo pathway components YAP and LATS. The phos-tag technology is particularly useful to quantitatively detect protein phosphorylation even without a phosphospecific antibody. We use HEK293A cells as an example, but the protocols can be applied to other cell lines and tissues. For complete details on the use and execution of this profile, please refer to Luo et al. (2020). Elsevier 2022-01-17 /pmc/articles/PMC9076959/ /pubmed/35535164 http://dx.doi.org/10.1016/j.xpro.2021.101102 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Luo, Min
Hu, Yuan
Wei, Xiawei
Guan, Kun-Liang
Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells
title Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells
title_full Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells
title_fullStr Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells
title_full_unstemmed Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells
title_short Protocols for measuring phosphorylation, subcellular localization, and kinase activity of Hippo pathway components YAP and LATS in cultured cells
title_sort protocols for measuring phosphorylation, subcellular localization, and kinase activity of hippo pathway components yap and lats in cultured cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076959/
https://www.ncbi.nlm.nih.gov/pubmed/35535164
http://dx.doi.org/10.1016/j.xpro.2021.101102
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