Cargando…

Purification of mouse axoplasmic proteins from dorsal root ganglia nerves for proteomics analysis

The study of neuronal signaling ex vivo requires the identification of the proteins that are represented within the neuronal axoplasm. Here, we describe a detailed protocol to isolate the axoplasm of peripheral and central axonal branches of sciatic dorsal root ganglia neurons in mice. The axoplasm...

Descripción completa

Detalles Bibliográficos
Autores principales: Kong, Guiping, Zhou, Luming, Freiwald, Anja, Shkura, Kirill, Di Giovanni, Simone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076968/
https://www.ncbi.nlm.nih.gov/pubmed/35535163
http://dx.doi.org/10.1016/j.xpro.2022.101166